A court in the Indian capital, Delhi, is set to begin hearing evidence from witnesses in the case of the gang rape and murder of a 23-year-old physiotherapy student in December.
Five of the accused men have arrived at the specially convened fast-track court. They have pleaded not guilty.
If convicted, they could face the death penalty. A sixth suspect is to be tried by a juvenile court.
The assault outraged India and sparked a debate about treatment of women.
The first of 80 witnesses to be produced by the prosecution is due to take the stand in the court on Tuesday.
All reporting of proceedings inside the courtroom have been banned and lawyers cannot speak to reporters.
A key witness in the case will be the victim's male friend who was with her when she was brutally attacked on board a moving bus.
The student died in a Singapore hospital on 29 December from her internal injuries.
Her 28-year-old companion cannot be named for legal reasons. He arrived at the courthouse in a wheelchair, still unable to walk properly due to injuries he also allegedly suffered in the attack.
The five defendants facing trial on Tuesday are Ram Singh and his brother Mukesh, Pawan Gupta, Vinay Sharma and Akshay Thakur.
They face 13 charges, including murder, gang-rape, kidnapping and destruction of evidence.
Prosecutors say they have extensive forensic evidence, supported by the suspects' mobile-phone records and the testimony of the dying woman and her companion.
Defence lawyers are expected to argue that the forensic evidence has been fabricated and that the rush to prosecute has led to an unsafe trial.
On Sunday, Indian President Pranab Mukherjee signed a new anti-rape law which has increased the minimum sentence for those convicted of gang-rape and allows for the death penalty to be used in extreme cases.
But women's groups have come out against the new law, saying that marital rape and sexual assaults by Indian security forces in conflict zones should have been included.