The EU plans to open an office in the rebel-held Libyan city of Benghazi to improve the flow of aid for the authorities there, EU foreign policy chief Catherine Ashton has said.
The office will help support civil society and the Interim Transitional National Council opposed to Libyan leader Col Muammar Gaddafi, she said.
Baroness Ashton was speaking to Euro MPs in a debate on EU foreign policy.
"There's much more we need to do," she told the European Parliament.
"Gaddafi must go from power and must end his regime," she said, earning some applause from MEPs.
She stressed the need for the EU to help the anti-Gaddafi rebels develop education, healthcare and border security.
Some party leaders in the parliament urged the EU to make a bigger commitment to supporting the pro-democracy movements in North Africa and the Middle East.
France and Italy have recognised the opposition Transitional National Council as the legitimate representative of the Libyan people.
The EU has frozen the assets of Col Gaddafi and members of his family, and banned the supply of arms, ammunition and any equipment that could be used for "internal repression".
The EU has also frozen assets held by Libya's sovereign wealth fund and central bank.