The US military has revoked access for two women at the heart of the scandal that led to Friday's resignation of General David Petraeus as CIA director.
Security clearance has been suspended for his ex-mistress, Paula Broadwell.
And Jill Kelley's pass at the Florida air force base where she organised social events has been frozen.
An FBI inquiry into Mrs Kelley's complaints of email harassment revealed that Mrs Broadwell and Gen Petraeus had been having an affair.
Mrs Broadwell, a 40-year-old married mother of two, had apparently sent the anonymous emails to Mrs Kelley warning her to stay away from Gen Petraeus.
Mrs Broadwell, who wrote a biography of Gen Petraeus, held a security clearance through her work as a reserve Army intelligence officer.
It is not uncommon for those under investigation to have such clearance suspended, officials say.An investigation is under way into how classified material ended up on the West Point graduate's computer and in her Charlotte, North Carolina home, which was searched by the FBI on Monday.
Meanwhile, a Pentagon spokesman said suspending Mrs Kelley's pass for MacDill Air Force Base was in the best interests of the local military community.
He confirmed the 37-year-old married mother of three would be able to enter the base, near Tampa, but only through the visitors' centre.
She had been described as a "social liaison" for the facility, which is the home of US Central Command.
In his first public comments on the scandal, US President Barack Obama told reporters on Wednesday he had seen no evidence that Gen Petraeus' extramarital affair compromised national security.
His resignation should be a "sidenote" to a distinguished career, said Mr Obama.
Gen Petraeus, who previously commanded the war effort in Iraq and Afghanistan, will testify on Friday to Congress about September's deadly attack on the US consulate in Libya.
He will not be questioned about his affair with Mrs Broadwell, said lawmakers.
Meanwhile, General John Allen, the top US commander in Afghanistan, said he plans to "fully co-operate" with an investigation into allegedly flirtatious emails he sent to Mrs Kelley, his lawyer said on Wednesday.
Adultery is illegal under military law, and Pentagon officials are sifting through thousands of pages of correspondence involving the married Gen Allen, 58, and Mrs Kelley.
He is said to have addressed her as "sweetheart" and "dear" in the communication, although the messages are not said to be overtly sexual.